UV-C irradiation compromises conidial germination, formation of appressoria, and induces transcription of three putative photolyase genes in the barley powdery mildew fungus, Blumeria graminis f. sp. hordei
M. Zhu, M. Riederer, и U. Hildebrandt. Fungal Biol, 123 (3):
218-230(2019)Zhu, Mo
Riederer, Markus
Hildebrandt, Ulrich
eng
Research Support, Non-U.S. Gov't
Netherlands
2019/02/26
Fungal Biol. 2019 Mar;123(3):218-230. doi: 10.1016/j.funbio.2018.12.002. Epub 2018 Dec 14..
DOI: 10.1016/j.funbio.2018.12.002
Аннотация
UV-C irradiation is known to compromise germination of Blumeria graminis conidia and to reduce powdery mildew infestation. However, only scarce information is available on the effects of UV-C irradiation on B. graminis appressorium formation. Applying a Formvar(R) resin-based in vitro system allowed for analyzing B. graminis germination and appressorium formation in absence of plant defense. UV-C irradiation more strongly affected the differentiation of appressoria than conidial germination. In vivo and in vitro, a single dose of 100 J m(-2) UV-C was sufficient to reduce germination to less than 20 % and decrease appressorium formation to values below 5 %. UV-C irradiation negatively affected pustule size and conidiation. White light-mediated photoreactivation was most effective immediately after UV-C irradiation, indicating that a prolonged phase of darkness after UV-C treatment increases the efficacy of B. graminis control. UV-C irradiation increased transcript levels of three putative B. graminis photolyase genes, while mere white light or blue light irradiation did not contribute to the transcriptional up-regulation. Thus, UV-C irradiation effectively controls B. graminis infestation and proliferation by restricting prepenetration processes. Nevertheless, photoreactivation plays an important role in UV-C-based powdery mildew control in crops and hence has to be considered for planning specific irradiation schedules.
Zhu, Mo
Riederer, Markus
Hildebrandt, Ulrich
eng
Research Support, Non-U.S. Gov't
Netherlands
2019/02/26
Fungal Biol. 2019 Mar;123(3):218-230. doi: 10.1016/j.funbio.2018.12.002. Epub 2018 Dec 14.
%0 Journal Article
%1 zhu2019irradiation
%A Zhu, M.
%A Riederer, M.
%A Hildebrandt, U.
%D 2019
%J Fungal Biol
%K & Ascomycota/*growth development/*radiation effects myOwn
%N 3
%P 218-230
%R 10.1016/j.funbio.2018.12.002
%T UV-C irradiation compromises conidial germination, formation of appressoria, and induces transcription of three putative photolyase genes in the barley powdery mildew fungus, Blumeria graminis f. sp. hordei
%U https://www.ncbi.nlm.nih.gov/pubmed/30798877
%V 123
%X UV-C irradiation is known to compromise germination of Blumeria graminis conidia and to reduce powdery mildew infestation. However, only scarce information is available on the effects of UV-C irradiation on B. graminis appressorium formation. Applying a Formvar(R) resin-based in vitro system allowed for analyzing B. graminis germination and appressorium formation in absence of plant defense. UV-C irradiation more strongly affected the differentiation of appressoria than conidial germination. In vivo and in vitro, a single dose of 100 J m(-2) UV-C was sufficient to reduce germination to less than 20 % and decrease appressorium formation to values below 5 %. UV-C irradiation negatively affected pustule size and conidiation. White light-mediated photoreactivation was most effective immediately after UV-C irradiation, indicating that a prolonged phase of darkness after UV-C treatment increases the efficacy of B. graminis control. UV-C irradiation increased transcript levels of three putative B. graminis photolyase genes, while mere white light or blue light irradiation did not contribute to the transcriptional up-regulation. Thus, UV-C irradiation effectively controls B. graminis infestation and proliferation by restricting prepenetration processes. Nevertheless, photoreactivation plays an important role in UV-C-based powdery mildew control in crops and hence has to be considered for planning specific irradiation schedules.
@article{zhu2019irradiation,
abstract = {UV-C irradiation is known to compromise germination of Blumeria graminis conidia and to reduce powdery mildew infestation. However, only scarce information is available on the effects of UV-C irradiation on B. graminis appressorium formation. Applying a Formvar(R) resin-based in vitro system allowed for analyzing B. graminis germination and appressorium formation in absence of plant defense. UV-C irradiation more strongly affected the differentiation of appressoria than conidial germination. In vivo and in vitro, a single dose of 100 J m(-2) UV-C was sufficient to reduce germination to less than 20 % and decrease appressorium formation to values below 5 %. UV-C irradiation negatively affected pustule size and conidiation. White light-mediated photoreactivation was most effective immediately after UV-C irradiation, indicating that a prolonged phase of darkness after UV-C treatment increases the efficacy of B. graminis control. UV-C irradiation increased transcript levels of three putative B. graminis photolyase genes, while mere white light or blue light irradiation did not contribute to the transcriptional up-regulation. Thus, UV-C irradiation effectively controls B. graminis infestation and proliferation by restricting prepenetration processes. Nevertheless, photoreactivation plays an important role in UV-C-based powdery mildew control in crops and hence has to be considered for planning specific irradiation schedules.},
added-at = {2024-02-15T15:08:22.000+0100},
author = {Zhu, M. and Riederer, M. and Hildebrandt, U.},
biburl = {https://www.bibsonomy.org/bibtex/23a1ec92b479e7e72d4ac9da102d05022/jvsi_all},
doi = {10.1016/j.funbio.2018.12.002},
interhash = {e046c3d5978b068ae311ef611eca6be1},
intrahash = {3a1ec92b479e7e72d4ac9da102d05022},
issn = {1878-6146 (Print)},
journal = {Fungal Biol},
keywords = {& Ascomycota/*growth development/*radiation effects myOwn},
note = {Zhu, Mo
Riederer, Markus
Hildebrandt, Ulrich
eng
Research Support, Non-U.S. Gov't
Netherlands
2019/02/26
Fungal Biol. 2019 Mar;123(3):218-230. doi: 10.1016/j.funbio.2018.12.002. Epub 2018 Dec 14.},
number = 3,
pages = {218-230},
timestamp = {2024-02-15T15:08:22.000+0100},
title = {UV-C irradiation compromises conidial germination, formation of appressoria, and induces transcription of three putative photolyase genes in the barley powdery mildew fungus, Blumeria graminis f. sp. hordei},
type = {Journal Article},
url = {https://www.ncbi.nlm.nih.gov/pubmed/30798877},
volume = 123,
year = 2019
}