Potassium is an inevitable component of plant life, and potassium channels play a pivotal role in plant growth and development. The role of potassium and of K+ channels in plant cell division and cell-cycle progression, however, has not been determined so far. K+ channel blocker studies with synchronized tobacco BY-2 cells revealed that K+ uptake is required for proper cell-cycle progression during the transition from G(1) to S phase. Electrophysiological studies (patch-clamp and voltage-clamp techniques) showed a cell-cycle dependency of K+ channel activities and reduced driving force for K+ uptake in dividing cells. Among the four Shaker-like K+ channel genes expressed in BY-2 cells, NKT1 represents an inwardly rectifying K+ channel that mediates K+ uptake. NKT1 is transcriptionally induced during G(1) phase, while transcripts of the outward-rectifier NTORK1 dominate S phase. Elongating BY-2 cells appeared hyperpolarized (-101 +/- 11 mV), and had elevated osmotic pressure and approximately twice the turgor pressure when compared with depolarized (-64 +/- 8 mV) dividing cells. This indicates that cells have to gain a threshold K+ level to re-enter the cell cycle. Based on these findings, turgor regulation through modulation of K+ channel density in plant cell division and cell-cycle progression is discussed.
%0 Journal Article
%1 RN1147
%A Sano, T.
%A Becker, D.
%A Ivashikina, N.
%A Wegner, L. H.
%A Zimmermann, U.
%A Roelfsema, M. R. G.
%A Nagata, T.
%A Hedrich, R.
%D 2007
%J Plant Journal
%K cell cycle myOwn
%N 3
%P 401-413
%R 10.1111/j.1365-313X.2007.03071.x
%T Plant cells must pass a K
threshold to re-enter the cell cycle
%U /brokenurl#<Go to ISI>://WOS:000245945300003
%V 50
%X Potassium is an inevitable component of plant life, and potassium channels play a pivotal role in plant growth and development. The role of potassium and of K+ channels in plant cell division and cell-cycle progression, however, has not been determined so far. K+ channel blocker studies with synchronized tobacco BY-2 cells revealed that K+ uptake is required for proper cell-cycle progression during the transition from G(1) to S phase. Electrophysiological studies (patch-clamp and voltage-clamp techniques) showed a cell-cycle dependency of K+ channel activities and reduced driving force for K+ uptake in dividing cells. Among the four Shaker-like K+ channel genes expressed in BY-2 cells, NKT1 represents an inwardly rectifying K+ channel that mediates K+ uptake. NKT1 is transcriptionally induced during G(1) phase, while transcripts of the outward-rectifier NTORK1 dominate S phase. Elongating BY-2 cells appeared hyperpolarized (-101 +/- 11 mV), and had elevated osmotic pressure and approximately twice the turgor pressure when compared with depolarized (-64 +/- 8 mV) dividing cells. This indicates that cells have to gain a threshold K+ level to re-enter the cell cycle. Based on these findings, turgor regulation through modulation of K+ channel density in plant cell division and cell-cycle progression is discussed.
@article{RN1147,
abstract = {Potassium is an inevitable component of plant life, and potassium channels play a pivotal role in plant growth and development. The role of potassium and of K+ channels in plant cell division and cell-cycle progression, however, has not been determined so far. K+ channel blocker studies with synchronized tobacco BY-2 cells revealed that K+ uptake is required for proper cell-cycle progression during the transition from G(1) to S phase. Electrophysiological studies (patch-clamp and voltage-clamp techniques) showed a cell-cycle dependency of K+ channel activities and reduced driving force for K+ uptake in dividing cells. Among the four Shaker-like K+ channel genes expressed in BY-2 cells, NKT1 represents an inwardly rectifying K+ channel that mediates K+ uptake. NKT1 is transcriptionally induced during G(1) phase, while transcripts of the outward-rectifier NTORK1 dominate S phase. Elongating BY-2 cells appeared hyperpolarized (-101 +/- 11 mV), and had elevated osmotic pressure and approximately twice the turgor pressure when compared with depolarized (-64 +/- 8 mV) dividing cells. This indicates that cells have to gain a threshold K+ level to re-enter the cell cycle. Based on these findings, turgor regulation through modulation of K+ channel density in plant cell division and cell-cycle progression is discussed.},
added-at = {2024-02-14T14:38:32.000+0100},
author = {Sano, T. and Becker, D. and Ivashikina, N. and Wegner, L. H. and Zimmermann, U. and Roelfsema, M. R. G. and Nagata, T. and Hedrich, R.},
biburl = {https://www.bibsonomy.org/bibtex/234fbbc19dbba27ebe81e37917005ccc8/rainerhedrich_2},
doi = {10.1111/j.1365-313X.2007.03071.x},
interhash = {518447716c59649dc6d1a202c9240f79},
intrahash = {34fbbc19dbba27ebe81e37917005ccc8},
issn = {0960-7412},
journal = {Plant Journal},
keywords = {cell cycle myOwn},
note = {160mj
Times Cited:58
Cited References Count:47},
number = 3,
pages = {401-413},
timestamp = {2024-02-14T14:38:32.000+0100},
title = {Plant cells must pass a K
threshold to re-enter the cell cycle},
type = {Journal Article},
url = {/brokenurl#<Go to ISI>://WOS:000245945300003},
volume = 50,
year = 2007
}