%0 Journal Article %1 RN1147 %A Sano, T. %A Becker, D. %A Ivashikina, N. %A Wegner, L. H. %A Zimmermann, U. %A Roelfsema, M. R. G. %A Nagata, T. %A Hedrich, R. %D 2007 %J Plant Journal %K cell cycle myOwn %N 3 %P 401-413 %R 10.1111/j.1365-313X.2007.03071.x %T Plant cells must pass a K threshold to re-enter the cell cycle %U /brokenurl#<Go to ISI>://WOS:000245945300003 %V 50 %X Potassium is an inevitable component of plant life, and potassium channels play a pivotal role in plant growth and development. The role of potassium and of K+ channels in plant cell division and cell-cycle progression, however, has not been determined so far. K+ channel blocker studies with synchronized tobacco BY-2 cells revealed that K+ uptake is required for proper cell-cycle progression during the transition from G(1) to S phase. Electrophysiological studies (patch-clamp and voltage-clamp techniques) showed a cell-cycle dependency of K+ channel activities and reduced driving force for K+ uptake in dividing cells. Among the four Shaker-like K+ channel genes expressed in BY-2 cells, NKT1 represents an inwardly rectifying K+ channel that mediates K+ uptake. NKT1 is transcriptionally induced during G(1) phase, while transcripts of the outward-rectifier NTORK1 dominate S phase. Elongating BY-2 cells appeared hyperpolarized (-101 +/- 11 mV), and had elevated osmotic pressure and approximately twice the turgor pressure when compared with depolarized (-64 +/- 8 mV) dividing cells. This indicates that cells have to gain a threshold K+ level to re-enter the cell cycle. Based on these findings, turgor regulation through modulation of K+ channel density in plant cell division and cell-cycle progression is discussed.

@article{RN1147, abstract = {Potassium is an inevitable component of plant life, and potassium channels play a pivotal role in plant growth and development. The role of potassium and of K+ channels in plant cell division and cell-cycle progression, however, has not been determined so far. K+ channel blocker studies with synchronized tobacco BY-2 cells revealed that K+ uptake is required for proper cell-cycle progression during the transition from G(1) to S phase. Electrophysiological studies (patch-clamp and voltage-clamp techniques) showed a cell-cycle dependency of K+ channel activities and reduced driving force for K+ uptake in dividing cells. Among the four Shaker-like K+ channel genes expressed in BY-2 cells, NKT1 represents an inwardly rectifying K+ channel that mediates K+ uptake. NKT1 is transcriptionally induced during G(1) phase, while transcripts of the outward-rectifier NTORK1 dominate S phase. Elongating BY-2 cells appeared hyperpolarized (-101 +/- 11 mV), and had elevated osmotic pressure and approximately twice the turgor pressure when compared with depolarized (-64 +/- 8 mV) dividing cells. This indicates that cells have to gain a threshold K+ level to re-enter the cell cycle. Based on these findings, turgor regulation through modulation of K+ channel density in plant cell division and cell-cycle progression is discussed.}, added-at = {2024-02-14T14:38:32.000+0100}, author = {Sano, T. and Becker, D. and Ivashikina, N. and Wegner, L. H. and Zimmermann, U. and Roelfsema, M. R. G. and Nagata, T. and Hedrich, R.}, biburl = {https://www.bibsonomy.org/bibtex/234fbbc19dbba27ebe81e37917005ccc8/rainerhedrich_2}, doi = {10.1111/j.1365-313X.2007.03071.x}, interhash = {518447716c59649dc6d1a202c9240f79}, intrahash = {34fbbc19dbba27ebe81e37917005ccc8}, issn = {0960-7412}, journal = {Plant Journal}, keywords = {cell cycle myOwn}, note = {160mj Times Cited:58 Cited References Count:47}, number = 3, pages = {401-413}, timestamp = {2024-02-14T14:38:32.000+0100}, title = {Plant cells must pass a K threshold to re-enter the cell cycle}, type = {Journal Article}, url = {/brokenurl#<Go to ISI>://WOS:000245945300003}, volume = 50, year = 2007 }