%0 Journal Article %1 WOS:000254632300015 %A Fontes, Adriana %A Fernandes, Heloise P %A Thomaz, Andre A De %A Barbosa, Luiz C %A Barjas-Castro, Maria L %A Cesar, Carlos L %C 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98225 USA %D 2008 %I SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS %J JOURNAL OF BIOMEDICAL OPTICS %K adhesion; agglutination; blood cell; membrane potential} red tweezers; viscosity; zeta {optical %N 1 %R 10.1117/1.2870108 %T Measuring electrical and mechanical properties of red blood cells with double optical tweezers %V 13 %X Red blood cell (RBC) aggregation in the blood stream is prevented by the zeta potential created by its negatively charged membrane. There are techniques, however, to decrease the zeta potential and allow cell agglutination, which are the basis of most of antigen-antibody tests used in immunohematology. We propose the use of optical tweezers to measure membrane viscosity, adhesion, zeta potential, and the double layer thickness of charges (DLT) formed around the cell in an electrolytic solution. For the membrane viscosity experiment, we trap a bead attached to RBCs and measure the force to slide one RBC over the other as a function of the velocity. Adhesion is quantified by displacing two RBCs apart until disagglutination. The DLT is measured using the force on the bead attached to a single RBC in response to an applied voltage. The zeta potential is obtained by measuring the terminal velocity after releasing the RBC from the trap at the last applied voltage. We believe that the methodology proposed here can provide information about agglutination, help to improve the tests usually performed in transfusion services, and be applied for zeta potential measurements in other samples. (C) 2008 Society of Photo-Optical Instrumentation Engineers.

@article{WOS:000254632300015, abstract = {Red blood cell (RBC) aggregation in the blood stream is prevented by the zeta potential created by its negatively charged membrane. There are techniques, however, to decrease the zeta potential and allow cell agglutination, which are the basis of most of antigen-antibody tests used in immunohematology. We propose the use of optical tweezers to measure membrane viscosity, adhesion, zeta potential, and the double layer thickness of charges (DLT) formed around the cell in an electrolytic solution. For the membrane viscosity experiment, we trap a bead attached to RBCs and measure the force to slide one RBC over the other as a function of the velocity. Adhesion is quantified by displacing two RBCs apart until disagglutination. The DLT is measured using the force on the bead attached to a single RBC in response to an applied voltage. The zeta potential is obtained by measuring the terminal velocity after releasing the RBC from the trap at the last applied voltage. We believe that the methodology proposed here can provide information about agglutination, help to improve the tests usually performed in transfusion services, and be applied for zeta potential measurements in other samples. (C) 2008 Society of Photo-Optical Instrumentation Engineers.}, added-at = {2022-05-23T20:00:14.000+0200}, address = {1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98225 USA}, author = {Fontes, Adriana and Fernandes, Heloise P and Thomaz, Andre A De and Barbosa, Luiz C and Barjas-Castro, Maria L and Cesar, Carlos L}, biburl = {https://www.bibsonomy.org/bibtex/2656a05ae52d445ef584d597fab322b71/ppgfis_ufc_br}, doi = {10.1117/1.2870108}, interhash = {fa43062329c36aa5042f5524a02210a8}, intrahash = {656a05ae52d445ef584d597fab322b71}, issn = {1083-3668}, journal = {JOURNAL OF BIOMEDICAL OPTICS}, keywords = {adhesion; agglutination; blood cell; membrane potential} red tweezers; viscosity; zeta {optical}, number = 1, publisher = {SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS}, pubstate = {published}, timestamp = {2022-05-23T20:00:14.000+0200}, title = {Measuring electrical and mechanical properties of red blood cells with double optical tweezers}, tppubtype = {article}, volume = 13, year = 2008 }