%0 Journal Article %1 Werther.2002b %A Werther, K. %A Christensen, I. J. %A Nielsen, H. J. %D 2002 %J Scand.J.Clin.Lab Invest %K 80 A Adult Aged Blood Chemistry Coagulation Colorectal Count Endothelial Factor Factors Female Growth Humans Immunomagnetic Intercellular Leukocytes Lymphocytes Lymphokines Male Middle Monocytes Neoplasms Peptides Plasma Platelet Platelets Proteins Research Separation Signaling Time Vascular Whole analysis and blood cells immunology methods over protein %N 5 %P 343-350 %T Determination of vascular endothelial growth factor (VEGF) in circulating blood: significance of VEGF in various leucocytes and platelets %U PM:12387579 %V 62 %X AIM: The sources of increased vascular endothelial growth factor (VEGF) concentrations in peripheral blood from cancer patients are not known in detail. The aim of the present study was to evaluate correlations between the VEGF content in isolated leucocyte subpopulations and VEGF concentrations in plasma, serum and lysed whole blood. METHODS: In 51 colorectal cancer (CRC) patients, circulating T-lymphocytes, B-lymphocytes, monocytes, and granulocytes were isolated by means of immunomagnetic separation. Subsequently, the isolated cells were lysed and VEGF contents in the lysates were determined. In corresponding blood samples, automated complete blood count was performed, and the number of each cell type was correlated to VEGF concentrations in plasma, serum and lysed whole blood. Finally, the impact of increasing clotting time on the release of VEGF to serum was analysed. RESULTS: Isolated neutrophils contained considerable amounts of VEGF. In isolated lymphocytes and monocytes, VEGF

@article{Werther.2002b, abstract = {AIM: The sources of increased vascular endothelial growth factor (VEGF) concentrations in peripheral blood from cancer patients are not known in detail. The aim of the present study was to evaluate correlations between the VEGF content in isolated leucocyte subpopulations and VEGF concentrations in plasma, serum and lysed whole blood. METHODS: In 51 colorectal cancer (CRC) patients, circulating T-lymphocytes, B-lymphocytes, monocytes, and granulocytes were isolated by means of immunomagnetic separation. Subsequently, the isolated cells were lysed and VEGF contents in the lysates were determined. In corresponding blood samples, automated complete blood count was performed, and the number of each cell type was correlated to VEGF concentrations in plasma, serum and lysed whole blood. Finally, the impact of increasing clotting time on the release of VEGF to serum was analysed. RESULTS: Isolated neutrophils contained considerable amounts of VEGF. In isolated lymphocytes and monocytes, VEGF }, added-at = {2010-02-05T11:28:39.000+0100}, author = {Werther, K. and Christensen, I. J. and Nielsen, H. J.}, biburl = {https://www.bibsonomy.org/bibtex/282491c95d7cde814b746cfac7395eeb2/kanefendt}, interhash = {ed0d4c81589dd7e942dbfcb1179ff47a}, intrahash = {82491c95d7cde814b746cfac7395eeb2}, journal = {Scand.J.Clin.Lab Invest}, keywords = {80 A Adult Aged Blood Chemistry Coagulation Colorectal Count Endothelial Factor Factors Female Growth Humans Immunomagnetic Intercellular Leukocytes Lymphocytes Lymphokines Male Middle Monocytes Neoplasms Peptides Plasma Platelet Platelets Proteins Research Separation Signaling Time Vascular Whole analysis and blood cells immunology methods over protein}, number = 5, pages = {343-350}, timestamp = {2010-02-05T11:28:41.000+0100}, title = {Determination of vascular endothelial growth factor (VEGF) in circulating blood: significance of VEGF in various leucocytes and platelets}, url = {PM:12387579}, volume = 62, year = 2002 }