Article,

A Study on the Physio-Therapeutic Effects of Myrtle (Myrtus Communis L.) Leaf- and Eucalyptus Leaf-Extracts on the Proliferation of Diarrhea-Inducing Escherichia Coli, Shigella, and Salmonella In Broilers Chickens

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CENTRAL ASIAN JOURNAL OF MEDICAL AND NATURAL SCIENCE, 4 (4): 110-119 (June 2023)

Abstract

This study was conducted to investigate the effectiveness of graded concentrations of myrtle (Myrtus communis L.) leaf- and Eucalyptus leaf-extracts (MLE and ELE, respectively) on the proliferation of diarrhea-inducing Escherichia coli, Shigella, and Salmonella in broilers chickens. The present study included the use of three bacterial isolates for each of the abovementioned bacteria obtained from the General Teaching Veterinary Hospital, Al-Diwaniyah City, Iraq, which previously were recovered from diarrheal cases of broiler chickens. These bacterial isolates were subjected to the leaf extracts (aqueous or alcoholic) in agar-gel diffusion tests at four concentrations (25, 50, 75, and 100mg/ml) as six plates for each concentration. Two control groups (water or ethanol) were used as six plates each. The results showed significant (p<0.05) effects of all concentrations for both extracts in inhibiting the growth of all bacteria compared with that from the controls. In a brief, significant (p<0.05) levels of bacterial inhibition were reported and were the highest from the aqueous MLE against the bacterial growth at 75 and 100mg/ml exceeding the effects of ampicillin and ciprofloxacin. At the second level, the aqueous and alcoholic ELE at 100mg/ml surpassed the impacts of ampicillin and ciprofloxacin against the bacterial growth. The alcoholic MLE came at the third place in its effectiveness against the tested pathogenic bacteria. The herein study findings suggest high effects of all myrtle leaf- and Eucalyptus leaf-extracts on the proliferation of diarrhea-inducing Escherichia coli, Shigella, and Salmonella in broilers chickens with the most impacts generated from the aqueous extract of myrtle leaves.

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