Article,
Isolation and Identification of Fungi Contaminated with Some Local and Imported Raisins and the Possibility of Controlling One of the Toxin-Producing Fungi by Using Ganoderma Lucidium Filtrate and Calcium Carbonate Treatments
CENTRAL ASIAN JOURNAL OF MEDICAL AND NATURAL SCIENCE, 4 (3): 573-581 (June 2023)
Abstract
Samples were collected from the shops, and local markets in the city of Al- Diwaniyah for the period from (October – December 2022) and multiple species of fungi were isolated: Aspergillus niger, Aspergillus flavus, Aspergillus tubingensis, Aspergillus teereus, Penicillium natatum, Rhizopu stolanifer, Alternaria Alternata, Fusarium sp, Some significant differences were observed in the fungi isolated from raisins and currant the frequency percentage highest in the treatment of non-sterilized from sterilized. A.tubingensis diagnosed phenotypically using classification keys and molecularly diagnosed by PCR technique (polymerase chain reaction). The results of the HPLC technique observed that the highest concentration of Aflatoxin B1 was due to the filterate of Aspergillus tubingensis isolate of the currant sample, which equalize to 114.9 ppb. Ganoderma lucidium filtrate and calcium carbonate shown their potential to significantly reduce the radial activity and dry weight of the this fungal isolate, where the maximum inhibition rate was76.85% for dry weight for radial activity at 30% concentration to treated the interplay between G.lucidium filtrate and calcium carbonate, while the highest inhibition rate was76.66 % for dry weight at 30% for same treat.
