Article,
Applications of Flow Cytometry to Characterize Bacterial Physiological Responses
BioMed Research International, (2014)
Abstract
Although reports of flow cytometry (FCM) applied to bacterial analysis are increasing, studies of FCM related to human cells still vastly outnumber other reports. However, current advances in FCM combined with a new generation of cellular reporter probes have made this technique suitable for analyzing physiological responses in bacteria. We review how FCM has been applied to characterize distinct physiological conditions in bacteria including responses to antibiotics and other cytotoxic chemicals and physical factors, pathogen-host interactions, cell differentiation during biofilm formation, and the mechanisms governing development pathways such as sporulation. Since FCM is suitable for performing studies at the single-cell level, we describe how this powerful technique has yielded invaluable information about the heterogeneous distribution of differently and even specialized responding cells and how it may help to provide insights about how cell interaction takes place in complex structures, such as those that prevail in bacterial biofilms."/><meta name="citation_fulltext_html_url" content="https://www.hindawi.com/journals/bmri/2014/461941/"/><meta name="citation_pdf_url" content="https://downloads.hindawi.com/journals/bmri/2014/461941.pdf"/><meta name="citation_xml_url" content="https://downloads.hindawi.com/journals/bmri/2014/461941.xml"/><meta name="dc.creator" content=Ämbriz-Aviña, Verónica"/><meta name="dc.creator" content="Contreras-Garduño, Jorge A."/><meta name="dc.creator" content="Pedraza-Reyes, Mario"/><meta name="dc.title" content=Äpplications of Flow Cytometry to Characterize Bacterial Physiological Responses"/><meta name="dc.description" content=Älthough reports of flow cytometry (FCM) applied to bacterial analysis are increasing, studies of FCM related to human cells still vastly outnumber other reports. However, current advances in FCM combined with a new generation of cellular reporter probes have made this technique suitable for analyzing physiological responses in bacteria. We review how FCM has been applied to characterize distinct physiological conditions in bacteria including responses to antibiotics and other cytotoxic chemicals and physical factors, pathogen-host interactions, cell differentiation during biofilm formation, and the mechanisms governing development pathways such as sporulation. Since FCM is suitable for performing studies at the single-cell level, we describe how this powerful technique has yielded invaluable information about the heterogeneous distribution of differently and even specialized responding cells and how it may help to provide insights about how cell interaction takes place in complex structures, such as those that prevail in bacterial biofilms."/><meta name="dc.publisher" content="Hindawi"/><meta name="dc.format" content="text/html"/><meta name="dc.language" content="en"/><meta name="dc.identifier" content="https://doi.org/10.1155/2014/461941"/><meta name="dc.type" content="Review Article"/><meta name="dc.date" content="2014/09/09"/><meta name="dcterms.issued" content="2014/09/09"/><meta name="prism.publicationDate" content="2014/09/09"/><meta name="prism.volume" content="2014"/><meta name="prism.section" content="Review Article"/><meta name="prism.doi" content="https://doi.org/10.1155/2014/461941"/><meta name="prism.issn" content="2314-6133"/><meta name="dc.source" content="BioMed Research International"/><meta name="prism.publicationName" content="BioMed Research International"/><meta name=äuthors" content="Verónica Ambriz-Aviña | Jorge A. Contreras-Garduño | Mario Pedraza-Reyes"/><meta name=Äuthor" content="Hindawi"/><meta name="robots" content="index"/><meta name="google-site-verification" content=ÄxEuDsL7vXGOxRe53-uFhOk2ODN0bbXMeuBy6Pfq4ww"/><script src="https://cdn.cookielaw.org/scripttemplates/otSDKStub.js" type="text/javascript" charset=ÜTF-8" data-domain-script="fbafd62a-0a4e-4f7b-a04d-56f57fa3d716"></script><link href="https://static-01.hindawi.com/next_assets/qodxNOfMTKPqd5jUhs9lE/static/lib/basictable.css" rel="stylesheet" media="none" class="next-head"/><link rel="stylesheet" href="https://static-01.hindawi.com/next_assets/qodxNOfMTKPqd5jUhs9lE/static/lib/owl.carousel.min.css" media="none" class="next-head"/><link rel="stylesheet" href="https://static-01.hindawi.com/next_assets/qodxNOfMTKPqd5jUhs9lE/static/lib/owl.theme.default.min.css" media="none" class="next-head"/><script src="https://static-01.hindawi.com/next_assets/qodxNOfMTKPqd5jUhs9lE/static/lib/jquery.js" class="next-head"></script><link rel="stylesheet" type="text/css" href="https://cdn.bibblio.org/rcm/4.18/bib-related-content.min.css" media="none" class="next-head"/><script src="https://cdn.bibblio.org/rcm/4.18/bib-related-content.min.js" class="next-head"></script><link rel="stylesheet" type="text/css" href="https://cdn.bibblio.org/rcm/4.18/bib-related-content.min.css" media="none" class="next-head
