%0 Journal Article %1 aires-de-sousa_high_2006 %A Aires-de-Sousa, M %A Boye, K %A de Lencastre, H %A Deplano, A %A Enright, M C %A Etienne, J %A Friedrich, A %A Harmsen, D %A Holmes, A %A Huijsdens, X W %A Kearns, A M %A Mellmann, A %A Meugnier, H %A Rasheed, J K %A Spalburg, E %A Strommenger, B %A Struelens, M J %A Tenover, F C %A Thomas, J %A Vogel, U %A Westh, H %A Xu, J %A Witte, W %D 2006 %J Journal of Clinical Microbiology %K A, Analysis, Bacterial Bacterial, Humans, Infections, Laboratories, Observer Protein Reproducibility Results, Sequence Software, Staphylococcal Staphylococcus Techniques, Typing Variation, aureus of {DNA}, %N 2 %P 619--621 %R 10.1128/JCM.44.2.619-621.2006 %T High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study %U http://www.ncbi.nlm.nih.gov/pubmed/16455927 %V 44 %X Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100\% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.

@article{aires-de-sousa_high_2006, abstract = {Current {DNA} amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, {DNA} sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100\% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus {DNA} samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.}, added-at = {2011-06-24T11:21:47.000+0200}, author = {{Aires-de-Sousa}, M and Boye, K and de Lencastre, H and Deplano, A and Enright, M C and Etienne, J and Friedrich, A and Harmsen, D and Holmes, A and Huijsdens, X W and Kearns, A M and Mellmann, A and Meugnier, H and Rasheed, J K and Spalburg, E and Strommenger, B and Struelens, M J and Tenover, F C and Thomas, J and Vogel, U and Westh, H and Xu, J and Witte, W}, biburl = {https://www.bibsonomy.org/bibtex/2b623829639fb8748aa7c57f022666b3a/ag_vogel}, doi = {10.1128/JCM.44.2.619-621.2006}, interhash = {900a4ec6dce0b62a57295564d99c68ea}, intrahash = {b623829639fb8748aa7c57f022666b3a}, issn = {0095-1137}, journal = {Journal of Clinical Microbiology}, keywords = {A, Analysis, Bacterial Bacterial, Humans, Infections, Laboratories, Observer Protein Reproducibility Results, Sequence Software, Staphylococcal Staphylococcus Techniques, Typing Variation, aureus of {DNA},}, month = feb, note = {{PMID:} 16455927}, number = 2, pages = {619--621}, timestamp = {2011-06-24T11:21:54.000+0200}, title = {High interlaboratory reproducibility of {DNA} sequence-based typing of bacteria in a multicenter study}, url = {http://www.ncbi.nlm.nih.gov/pubmed/16455927}, volume = 44, year = 2006 }