Mammalian axons are specialized for transmitting action potentials to targets within the central and peripheral nervous system. A growing body of evidence suggests that, besides signal conduction, axons play essential roles in neural information processing, and their malfunctions are common hallmarks of neurodegenerative diseases. The technologies available to study axonal function and structure integrally limit the comprehension of axon neurobiology. High-density microelectrode arrays (HD-MEAs) allow for accessing axonal action potentials at high spatiotemporal resolution, but provide no insights on axonal morphology. Here, we demonstrate a method for electrical visualization of axonal morphologies based on extracellular action potentials recorded from cortical and motor neurons using HD-MEAs. The method enabled us to reconstruct up to 5-cm-long axonal arbors and directly monitor axonal conduction across thousands of recording sites. We reconstructed 1.86 m of cortical and spinal axons in total and found specific features in their structure and function.
%0 Journal Article
%1 radivojevic2023functional
%A Radivojevic, Milos
%A Rostedt Punga, Anna
%D 2023
%E Radisic, Milica
%E Poirazi, Panayiota
%E Radisic, Milica
%I eLife Sciences Publications, Ltd
%J eLife
%K biology conduction_dynamics cortex_activity neuron neuroscience spinal_axons
%P e86512
%R 10.7554/eLife.86512
%T Functional imaging of conduction dynamics in cortical and spinal axons
%U https://doi.org/10.7554/eLife.86512
%V 12
%X Mammalian axons are specialized for transmitting action potentials to targets within the central and peripheral nervous system. A growing body of evidence suggests that, besides signal conduction, axons play essential roles in neural information processing, and their malfunctions are common hallmarks of neurodegenerative diseases. The technologies available to study axonal function and structure integrally limit the comprehension of axon neurobiology. High-density microelectrode arrays (HD-MEAs) allow for accessing axonal action potentials at high spatiotemporal resolution, but provide no insights on axonal morphology. Here, we demonstrate a method for electrical visualization of axonal morphologies based on extracellular action potentials recorded from cortical and motor neurons using HD-MEAs. The method enabled us to reconstruct up to 5-cm-long axonal arbors and directly monitor axonal conduction across thousands of recording sites. We reconstructed 1.86 m of cortical and spinal axons in total and found specific features in their structure and function.
@article{radivojevic2023functional,
abstract = {Mammalian axons are specialized for transmitting action potentials to targets within the central and peripheral nervous system. A growing body of evidence suggests that, besides signal conduction, axons play essential roles in neural information processing, and their malfunctions are common hallmarks of neurodegenerative diseases. The technologies available to study axonal function and structure integrally limit the comprehension of axon neurobiology. High-density microelectrode arrays (HD-MEAs) allow for accessing axonal action potentials at high spatiotemporal resolution, but provide no insights on axonal morphology. Here, we demonstrate a method for electrical visualization of axonal morphologies based on extracellular action potentials recorded from cortical and motor neurons using HD-MEAs. The method enabled us to reconstruct up to 5-cm-long axonal arbors and directly monitor axonal conduction across thousands of recording sites. We reconstructed 1.86 m of cortical and spinal axons in total and found specific features in their structure and function.},
added-at = {2023-10-15T12:51:52.000+0200},
article_type = {journal},
author = {Radivojevic, Milos and Rostedt Punga, Anna},
biburl = {https://www.bibsonomy.org/bibtex/2925a8455f64522ebf0d4662990d8b685/tabularii},
citation = {eLife 2023;12:e86512},
doi = {10.7554/eLife.86512},
editor = {Radisic, Milica and Poirazi, Panayiota and Radisic, Milica},
interhash = {cc5bfcdf87eccb43c4a2ac0fd8880c70},
intrahash = {925a8455f64522ebf0d4662990d8b685},
issn = {2050-084X},
journal = {eLife},
keywords = {biology conduction_dynamics cortex_activity neuron neuroscience spinal_axons},
month = aug,
pages = {e86512},
pub_date = {2023-08-22},
publisher = {eLife Sciences Publications, Ltd},
timestamp = {2023-10-15T12:51:52.000+0200},
title = {Functional imaging of conduction dynamics in cortical and spinal axons},
url = {https://doi.org/10.7554/eLife.86512},
volume = 12,
year = 2023
}