Kawai M, Hussain M, and Orchard CH. Am J Heart Circ Physiol 277:
H603-H609, 1999 developed a technique to detubulate rat ventricular
myocytes using formamide and showed that detubulation results in
a decrease in cell capacitance, Ca$^2+$ current density, and
Ca$^2+$ transient amplitude. We have investigated the mechanism
of this detubulation and possible direct effects of formamide. Staining
ventricular cells with di-8-ANEPPS showed that the t tubule membranes
remain inside the cell after detubulation; trapping of FITC-labeled
dextran within the t tubules showed that detubulation occurs during
formamide washout and that the t tubules appear to reseal within
the cell. Detubulation had no effect on the microtubule network but
resulted in loss of synchronous Ca$^2+$ release on electrical
stimulation. In contrast, formamide treatment of atrial cells did
not significantly change cell capacitance, Ca$^2+$ current amplitude,
action potential configuration, the Ca$^2+$ transient or the
response of the Ca$^2+$ transient to isoprenaline. We conclude
that formamide washout induces detubulation of single rat ventricular
myocytes, leaving the t tubules within the cell, but without direct
effects on cell proteins that might alter cell function.
%0 Journal Article
%1 Bret_2002_H1720
%A Brette, Fabien
%A Komukai, Kimiaki
%A Orchard, Clive H
%D 2002
%J Am. J. Physiol. Heart Circ. Physiol.
%K 12234828 Action Agents, Animals, Atria, Calcium, Cardiotonic Compounds, Dyes, Electrophysiology, Fibers, Fluorescent Formamides, Gov't, Heart Isoproterenol, Microtubules, Muscle Myocardium, Non-U.S. Potentials, Pyridinium Rats, Research Sarcolemma, Support,
%N 4
%P H1720-8
%R 10.1152/ajpheart.00347.2002
%T Validation of formamide as a detubulation agent in isolated rat cardiac
cells.
%U http://dx.doi.org/10.1152/ajpheart.00347.2002
%V 283
%X Kawai M, Hussain M, and Orchard CH. Am J Heart Circ Physiol 277:
H603-H609, 1999 developed a technique to detubulate rat ventricular
myocytes using formamide and showed that detubulation results in
a decrease in cell capacitance, Ca$^2+$ current density, and
Ca$^2+$ transient amplitude. We have investigated the mechanism
of this detubulation and possible direct effects of formamide. Staining
ventricular cells with di-8-ANEPPS showed that the t tubule membranes
remain inside the cell after detubulation; trapping of FITC-labeled
dextran within the t tubules showed that detubulation occurs during
formamide washout and that the t tubules appear to reseal within
the cell. Detubulation had no effect on the microtubule network but
resulted in loss of synchronous Ca$^2+$ release on electrical
stimulation. In contrast, formamide treatment of atrial cells did
not significantly change cell capacitance, Ca$^2+$ current amplitude,
action potential configuration, the Ca$^2+$ transient or the
response of the Ca$^2+$ transient to isoprenaline. We conclude
that formamide washout induces detubulation of single rat ventricular
myocytes, leaving the t tubules within the cell, but without direct
effects on cell proteins that might alter cell function.
@article{Bret_2002_H1720,
abstract = {Kawai M, Hussain M, and Orchard {CH}. Am J Heart Circ Physiol 277:
H603-H609, 1999 developed a technique to detubulate rat ventricular
myocytes using formamide and showed that detubulation results in
a decrease in cell capacitance, {C}a$^{2+}$ current density, and
{C}a$^{2+}$ transient amplitude. We have investigated the mechanism
of this detubulation and possible direct effects of formamide. Staining
ventricular cells with di-8-{ANEPPS} showed that the t tubule membranes
remain inside the cell after detubulation; trapping of {FITC}-labeled
dextran within the t tubules showed that detubulation occurs during
formamide washout and that the t tubules appear to reseal within
the cell. Detubulation had no effect on the microtubule network but
resulted in loss of synchronous {C}a$^{2+}$ release on electrical
stimulation. In contrast, formamide treatment of atrial cells did
not significantly change cell capacitance, {C}a$^{2+}$ current amplitude,
action potential configuration, the {C}a$^{2+}$ transient or the
response of the {C}a$^{2+}$ transient to isoprenaline. We conclude
that formamide washout induces detubulation of single rat ventricular
myocytes, leaving the t tubules within the cell, but without direct
effects on cell proteins that might alter cell function.},
added-at = {2009-06-03T11:20:58.000+0200},
author = {Brette, Fabien and Komukai, Kimiaki and Orchard, Clive H},
biburl = {https://www.bibsonomy.org/bibtex/273efefa83980333fb35b1996818b3493/hake},
description = {The whole bibliography file I use.},
doi = {10.1152/ajpheart.00347.2002},
file = {Bret_2002_H1720.pdf:Bret_2002_H1720.pdf:PDF},
interhash = {f425af2da98dd1a0096fce3a3e5bedb0},
intrahash = {73efefa83980333fb35b1996818b3493},
journal = {Am. J. Physiol. Heart Circ. Physiol.},
key = 40,
keywords = {12234828 Action Agents, Animals, Atria, Calcium, Cardiotonic Compounds, Dyes, Electrophysiology, Fibers, Fluorescent Formamides, Gov't, Heart Isoproterenol, Microtubules, Muscle Myocardium, Non-U.S. Potentials, Pyridinium Rats, Research Sarcolemma, Support,},
month = Oct,
number = 4,
pages = {H1720-8},
pii = {00347.2002},
timestamp = {2009-06-03T11:21:06.000+0200},
title = {Validation of formamide as a detubulation agent in isolated rat cardiac
cells.},
url = {http://dx.doi.org/10.1152/ajpheart.00347.2002},
volume = 283,
year = 2002
}