%0 Journal Article %1 Meis_1982_7704 %A Meissner, G. %A McKinley, D. %D 1982 %J J. Biol. Chem. %K 7085644 Active, Animals, Biological Chlorides, Concentration, Dogs, Electron, Gov't, Hydrogen-Ion Intracellular Kinetics, Membranes, Microscopy, Microsomes, Myocardium, P.H.S., Permeability, Potassium, Research Reticulum, Sarcoplasmic Sodium, Support, Transport, U.S. %N 13 %P 7704--7711 %T Permeability of canine cardiac sarcoplasmic reticulum vesicles to K$^+$, Na$^+$, H$^+$, and Cl$^-$. %V 257 %X Cardiac muscle sarcoplasmic reticulum appears to contain channel-like structures that render the membrane permeable to small univalent ions. Canine heart microsomes fractionated according to buoyant density were examined by Millipore filtration, light scattering, and membrane potential m easurements. Enzymatic analysis and measurement of D-glucose permeation and Na/Ca exchange systems indicated two membrane fractions suitable for the permeability studies, one enriched in surface membranes with a buoyant density of 1.04-1.11 (10-25\% sucrose) and one enriched in sarcoplasmic reticulum with a buoyant density of 1.13-1.15 (30-34\% sucrose). Surface membrane vesicles impermeable to 3Hsucrose were largely impermeable to K$^+$, Na$^+$, and Cl$^-$, while sarcoplasmic reticulum vesicles impermeable to 3Hsucrose were readily permeable to K$^+$, Na$^+$, H$^+$, and Cl$^-$. Sarcoplasmic reticulum vesicles were essentially impermeable to Ca$^2+$, Mg2+, choline+, gluconate-, 1,4-piperazinediethanesulfonic acid (Pipes-), and D-glucose. These results suggest that cardiac muscle sarcoplasmic reticulum contains structures that facilitate the movement of small univalent ions. A possible function of these putative ion-conducting structures may be to allow rapid ion fluxes to counter electrogenic Ca$^2+$ fluxes across sarcoplasmic reticulum during cardiac muscle contraction and relaxation.

@article{Meis_1982_7704, abstract = {Cardiac muscle sarcoplasmic reticulum appears to contain channel-like structures that render the membrane permeable to small univalent ions. Canine heart microsomes fractionated according to buoyant density were examined by Millipore filtration, light scattering, and membrane potential m easurements. Enzymatic analysis and measurement of D-glucose permeation and Na/Ca exchange systems indicated two membrane fractions suitable for the permeability studies, one enriched in surface membranes with a buoyant density of 1.04-1.11 (10-25\% sucrose) and one enriched in sarcoplasmic reticulum with a buoyant density of 1.13-1.15 (30-34\% sucrose). Surface membrane vesicles impermeable to [3H]sucrose were largely impermeable to {K}$^{+}$, {N}a$^{+}$, and {C}l$^{-}$, while sarcoplasmic reticulum vesicles impermeable to [3H]sucrose were readily permeable to {K}$^{+}$, {N}a$^{+}$, {H}$^+$, and {C}l$^{-}$. Sarcoplasmic reticulum vesicles were essentially impermeable to {C}a$^{2+}$, Mg2+, choline+, gluconate-, 1,4-piperazinediethanesulfonic acid (Pipes-), and D-glucose. These results suggest that cardiac muscle sarcoplasmic reticulum contains structures that facilitate the movement of small univalent ions. A possible function of these putative ion-conducting structures may be to allow rapid ion fluxes to counter electrogenic {C}a$^{2+}$ fluxes across sarcoplasmic reticulum during cardiac muscle contraction and relaxation.}, added-at = {2009-06-03T11:20:58.000+0200}, author = {Meissner, G. and McKinley, D.}, biburl = {https://www.bibsonomy.org/bibtex/26c320136a3b8893a64eb670de926c2a9/hake}, description = {The whole bibliography file I use.}, file = {Meis_1982_7704.pdf:Meis_1982_7704.pdf:PDF}, interhash = {71e13490c7749ed5f7c4767a3d1a966d}, intrahash = {6c320136a3b8893a64eb670de926c2a9}, journal = {J. Biol. Chem.}, keywords = {7085644 Active, Animals, Biological Chlorides, Concentration, Dogs, Electron, Gov't, Hydrogen-Ion Intracellular Kinetics, Membranes, Microscopy, Microsomes, Myocardium, P.H.S., Permeability, Potassium, Research Reticulum, Sarcoplasmic Sodium, Support, Transport, U.S.}, month = Jul, number = 13, pages = {7704--7711}, pmid = {7085644}, timestamp = {2009-06-03T11:21:22.000+0200}, title = {Permeability of canine cardiac sarcoplasmic reticulum vesicles to {K}$^{+}$, {N}a$^{+}$, {H}$^+$, and {C}l$^{-}$.}, volume = 257, year = 1982 }