Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront biologists with the challenge of selecting the best-suited approach for their particular research question. Here, we provide guidance on how to use SRM techniques advantageously for investigating cellular structures and dynamics to promote new discoveries.
%0 Journal Article
%1 schermelleh2019superresolution
%A Schermelleh, Lothar
%A Ferrand, Alexia
%A Huser, Thomas
%A Eggeling, Christian
%A Sauer, Markus
%A Biehlmaier, Oliver
%A Drummen, Gregor P. C.
%D 2019
%J Nature Cell Biology
%K home sauer
%N 1
%P 72--84
%R 10.1038/s41556-018-0251-8
%T Super-resolution microscopy demystified
%U https://doi.org/10.1038/s41556-018-0251-8
%V 21
%X Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront biologists with the challenge of selecting the best-suited approach for their particular research question. Here, we provide guidance on how to use SRM techniques advantageously for investigating cellular structures and dynamics to promote new discoveries.
@article{schermelleh2019superresolution,
abstract = {Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront biologists with the challenge of selecting the best-suited approach for their particular research question. Here, we provide guidance on how to use SRM techniques advantageously for investigating cellular structures and dynamics to promote new discoveries.},
added-at = {2019-01-09T09:42:56.000+0100},
author = {Schermelleh, Lothar and Ferrand, Alexia and Huser, Thomas and Eggeling, Christian and Sauer, Markus and Biehlmaier, Oliver and Drummen, Gregor P. C.},
biburl = {https://www.bibsonomy.org/bibtex/2493f7115604715387b0da409c90040f0/reichert},
doi = {10.1038/s41556-018-0251-8},
interhash = {db0b88a102ceb9d06a5527b5d3121bc0},
intrahash = {493f7115604715387b0da409c90040f0},
issn = {14764679},
journal = {Nature Cell Biology},
keywords = {home sauer},
number = 1,
pages = {72--84},
refid = {Schermelleh2019},
timestamp = {2019-07-25T11:03:27.000+0200},
title = {Super-resolution microscopy demystified},
url = {https://doi.org/10.1038/s41556-018-0251-8},
volume = 21,
year = 2019
}