Maternal-to-filial nutrition transfer is central to grain development and yield. nitrate transporter 1/peptide transporter (NRT1-PTR)-type transporters typically transport nitrate, peptides, and ions. Here, we report the identification of a maize (Zea mays) NRT1-PTR-type transporter that transports sucrose and glucose. The activity of this sugar transporter, named Sucrose and Glucose Carrier 1 (SUGCAR1), was systematically verified by tracer-labeled sugar uptake and serial electrophysiological studies including two-electrode voltage-clamp, non-invasive microelectrode ion flux estimation assays in Xenopus laevis oocytes and patch clamping in HEK293T cells. ZmSUGCAR1 is specifically expressed in the basal endosperm transfer layer and loss-of-function mutation of ZmSUGCAR1 caused significantly decreased sucrose and glucose contents and subsequent shrinkage of maize kernels. Notably, the ZmSUGCAR1 orthologs SbSUGCAR1 (from Sorghum bicolor) and TaSUGCAR1 (from Triticum aestivum) displayed similar sugar transport activities in oocytes, supporting the functional conservation of SUGCAR1 in closely related cereal species. Thus, the discovery of ZmSUGCAR1 uncovers a type of sugar transporter essential for grain development and opens potential avenues for genetic improvement of seed-filling and yield in maize and other grain crops.
%0 Journal Article
%1 yang2022sugar
%A Yang, B.
%A Wang, J.
%A Yu, M.
%A Zhang, M.
%A Zhong, Y.
%A Wang, T.
%A Liu, P.
%A Song, W.
%A Zhao, H.
%A Fastner, A.
%A Suter, M.
%A Rentsch, D.
%A Ludewig, U.
%A Jin, W.
%A Geiger, D.
%A Hedrich, R.
%A Braun, D. M.
%A Koch, K. E.
%A McCarty, D. R.
%A Wu, W. H.
%A Li, X.
%A Wang, Y.
%A Lai, J.
%D 2022
%J Plant Cell
%K Humans myOwn
%N 11
%P 4232-4254
%R 10.1093/plcell/koac256
%T The sugar transporter ZmSUGCAR1 of the nitrate transporter 1/peptide transporter family is critical for maize grain filling
%U https://www.ncbi.nlm.nih.gov/pubmed/36047828
%V 34
%X Maternal-to-filial nutrition transfer is central to grain development and yield. nitrate transporter 1/peptide transporter (NRT1-PTR)-type transporters typically transport nitrate, peptides, and ions. Here, we report the identification of a maize (Zea mays) NRT1-PTR-type transporter that transports sucrose and glucose. The activity of this sugar transporter, named Sucrose and Glucose Carrier 1 (SUGCAR1), was systematically verified by tracer-labeled sugar uptake and serial electrophysiological studies including two-electrode voltage-clamp, non-invasive microelectrode ion flux estimation assays in Xenopus laevis oocytes and patch clamping in HEK293T cells. ZmSUGCAR1 is specifically expressed in the basal endosperm transfer layer and loss-of-function mutation of ZmSUGCAR1 caused significantly decreased sucrose and glucose contents and subsequent shrinkage of maize kernels. Notably, the ZmSUGCAR1 orthologs SbSUGCAR1 (from Sorghum bicolor) and TaSUGCAR1 (from Triticum aestivum) displayed similar sugar transport activities in oocytes, supporting the functional conservation of SUGCAR1 in closely related cereal species. Thus, the discovery of ZmSUGCAR1 uncovers a type of sugar transporter essential for grain development and opens potential avenues for genetic improvement of seed-filling and yield in maize and other grain crops.
@article{yang2022sugar,
abstract = {Maternal-to-filial nutrition transfer is central to grain development and yield. nitrate transporter 1/peptide transporter (NRT1-PTR)-type transporters typically transport nitrate, peptides, and ions. Here, we report the identification of a maize (Zea mays) NRT1-PTR-type transporter that transports sucrose and glucose. The activity of this sugar transporter, named Sucrose and Glucose Carrier 1 (SUGCAR1), was systematically verified by tracer-labeled sugar uptake and serial electrophysiological studies including two-electrode voltage-clamp, non-invasive microelectrode ion flux estimation assays in Xenopus laevis oocytes and patch clamping in HEK293T cells. ZmSUGCAR1 is specifically expressed in the basal endosperm transfer layer and loss-of-function mutation of ZmSUGCAR1 caused significantly decreased sucrose and glucose contents and subsequent shrinkage of maize kernels. Notably, the ZmSUGCAR1 orthologs SbSUGCAR1 (from Sorghum bicolor) and TaSUGCAR1 (from Triticum aestivum) displayed similar sugar transport activities in oocytes, supporting the functional conservation of SUGCAR1 in closely related cereal species. Thus, the discovery of ZmSUGCAR1 uncovers a type of sugar transporter essential for grain development and opens potential avenues for genetic improvement of seed-filling and yield in maize and other grain crops.},
added-at = {2024-02-15T15:08:22.000+0100},
author = {Yang, B. and Wang, J. and Yu, M. and Zhang, M. and Zhong, Y. and Wang, T. and Liu, P. and Song, W. and Zhao, H. and Fastner, A. and Suter, M. and Rentsch, D. and Ludewig, U. and Jin, W. and Geiger, D. and Hedrich, R. and Braun, D. M. and Koch, K. E. and McCarty, D. R. and Wu, W. H. and Li, X. and Wang, Y. and Lai, J.},
biburl = {https://www.bibsonomy.org/bibtex/226cb76adfca8df27659760a46a5fc7e5/jvsi_all},
doi = {10.1093/plcell/koac256},
interhash = {ad19152a6228813eed4cb21261955f00},
intrahash = {26cb76adfca8df27659760a46a5fc7e5},
issn = {1532-298X (Electronic)
1040-4651 (Print)
1040-4651 (Linking)},
journal = {Plant Cell},
keywords = {Humans myOwn},
note = {Yang, Bo
Wang, Jing
Yu, Miao
Zhang, Meiling
Zhong, Yanting
Wang, Tianyi
Liu, Peng
Song, Weibin
Zhao, Haiming
Fastner, Astrid
Suter, Marianne
Rentsch, Doris
Ludewig, Uwe
Jin, Weiwei
Geiger, Dietmar
Hedrich, Rainer
Braun, David M
Koch, Karen E
McCarty, Donald R
Wu, Wei-Hua
Li, Xuexian
Wang, Yi
Lai, Jinsheng
eng
Research Support, Non-U.S. Gov't
England
2022/09/02
Plant Cell. 2022 Oct 27;34(11):4232-4254. doi: 10.1093/plcell/koac256.},
number = 11,
pages = {4232-4254},
timestamp = {2024-02-15T15:08:22.000+0100},
title = {The sugar transporter ZmSUGCAR1 of the nitrate transporter 1/peptide transporter family is critical for maize grain filling},
type = {Journal Article},
url = {https://www.ncbi.nlm.nih.gov/pubmed/36047828},
volume = 34,
year = 2022
}