%0 Journal Article %1 gonzalezmontoroVps39InteractsTom402018a %A González Montoro, Ayelén %A Auffarth, Kathrin %A Hönscher, Carina %A Bohnert, Maria %A Becker, Thomas %A Warscheid, Bettina %A Reggiori, Fulvio %A van der Laan, Martin %A Fröhlich, Florian %A Ungermann, Christian %C United States %D 2018 %J Developmental cell %K & Adaptor Fusion,mitochondria,Mitochondria/*metabolism,Mitochondrial Membrane Membranes/*metabolism,Saccharomyces Phenomena,ERMES,membrane Physiological Proteins Proteins/genetics/*metabolism,Mitochondrial Proteins/genetics/*metabolism,Saccharomyces Transport Transport/genetics/*metabolism,Cell Vesicular cerevisiae cerevisiae/genetics/growth complex,Tom40,vacuole,Vacuoles/*metabolism,vCLAMP,Vps13,Vps39 contact development/*metabolism,to_read,TOM sites,Membrane %N 5 %P 621-636.e7 %R 10.1016/j.devcel.2018.05.011 %T Vps39 Interacts with Tom40 to Establish One of Two Functionally Distinct Vacuole-Mitochondria Contact Sites. %V 45 %X The extensive subcellular network of membrane contact sites plays central roles in organelle biogenesis and communication, yet the precise contributions of the involved machineries remain largely enigmatic. The yeast vacuole forms a membrane contact site with mitochondria, called vacuolar and mitochondrial patch (vCLAMP). Formation of vCLAMPs involves the vacuolar Rab GTPase Ypt7 and the Ypt7-interacting Vps39 subunit of the HOPS tethering complex. Here, we uncover the general preprotein translocase of the outer membrane (TOM) subunit Tom40 as the direct binding partner of Vps39 on mitochondria. We identify Vps39 mutants defective in TOM binding, but functional for HOPS. Cells that cannot form vCLAMPs show reduced growth under stress conditions and impaired survival upon starvation. Unexpectedly, our mutant analysis revealed the existence of two functionally independent vacuole-mitochondria MCSs: one formed by the Ypt7-Vps39-Tom40 tether and a second one by Vps13-Mcp1, which is redundant with ER-mitochondrial contacts formed by ERMES.

@article{gonzalezmontoroVps39InteractsTom402018a, abstract = {The extensive subcellular network of membrane contact sites plays central roles in organelle biogenesis and communication, yet the precise contributions of the involved machineries remain largely enigmatic. The yeast vacuole forms a membrane contact site with mitochondria, called vacuolar and mitochondrial patch (vCLAMP). Formation of vCLAMPs involves the vacuolar Rab GTPase Ypt7 and the Ypt7-interacting Vps39 subunit of the HOPS tethering complex. Here, we uncover the general preprotein translocase of the outer membrane (TOM) subunit Tom40 as the direct binding partner of Vps39 on mitochondria. We identify Vps39 mutants defective in TOM binding, but functional for HOPS. Cells that cannot form vCLAMPs show reduced growth under stress conditions and impaired survival upon starvation. Unexpectedly, our mutant analysis revealed the existence of two functionally independent vacuole-mitochondria MCSs: one formed by the Ypt7-Vps39-Tom40 tether and a second one by Vps13-Mcp1, which is redundant with ER-mitochondrial contacts formed by ERMES.}, added-at = {2024-05-17T13:01:35.000+0200}, address = {United States}, author = {Gonz{\'a}lez Montoro, Ayel{\'e}n and Auffarth, Kathrin and H{\"o}nscher, Carina and Bohnert, Maria and Becker, Thomas and Warscheid, Bettina and Reggiori, Fulvio and {van der Laan}, Martin and Fr{\"o}hlich, Florian and Ungermann, Christian}, biburl = {https://www.bibsonomy.org/bibtex/20d1852b0c4611d07aff3714e0b6d574f/warscheidlab}, copyright = {Copyright {\copyright} 2018 Elsevier Inc. All rights reserved.}, doi = {10.1016/j.devcel.2018.05.011}, interhash = {7440c655530b1a927530e799c1a6083b}, intrahash = {0d1852b0c4611d07aff3714e0b6d574f}, issn = {1878-1551 1534-5807}, journal = {Developmental cell}, keywords = {& Adaptor Fusion,mitochondria,Mitochondria/*metabolism,Mitochondrial Membrane Membranes/*metabolism,Saccharomyces Phenomena,ERMES,membrane Physiological Proteins Proteins/genetics/*metabolism,Mitochondrial Proteins/genetics/*metabolism,Saccharomyces Transport Transport/genetics/*metabolism,Cell Vesicular cerevisiae cerevisiae/genetics/growth complex,Tom40,vacuole,Vacuoles/*metabolism,vCLAMP,Vps13,Vps39 contact development/*metabolism,to_read,TOM sites,Membrane}, langid = {english}, month = jun, number = 5, pages = {621-636.e7}, pmid = {29870720}, timestamp = {2024-05-17T13:01:35.000+0200}, title = {Vps39 {{Interacts}} with {{Tom40}} to {{Establish One}} of {{Two Functionally Distinct Vacuole-Mitochondria Contact Sites}}.}, volume = 45, year = 2018 }