Abstract
Multiphoton microscopy (MPM) has found a niche in the world of biological
imaging as the best noninvasive means of fluorescence microscopy
in tissue explants and living animals. Coupled with transgenic mouse
models of disease and 'smart' genetically encoded fluorescent indicators,
its use is now increasing exponentially. Properly applied, it is
capable of measuring calcium transients 500 mum deep in a mouse brain,
or quantifying blood flow by imaging shadows of blood cells as they
race through capillaries. With the multitude of possibilities afforded
by variations of nonlinear optics and localized photochemistry, it
is possible to image collagen fibrils directly within tissue through
nonlinear scattering, or release caged compounds in sub-femtoliter
volumes.
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