%0 Journal Article %1 noauthororeditor %A Saleh, Zainab Agab Altaee | Karama Tahreer Ahmed Altaee | Eman Naji %D 2023 %J CENTRAL ASIAN JOURNAL OF MEDICAL AND NATURAL SCIENCE %K Pyocyanin antibacterial efficacy production, %N 6 %P 988-994 %T Comparative Analysis of Pyocyanin Production and Antibacterial Efficacy on Diverse Culture %U https://cajmns.centralasianstudies.org/index.php/CAJMNS/article/view/2174/2215 %V 4 %X We investigated the impact of culture medium on pyocyanin production by Pseudomonas aeruginosa. Our study included variations in media, temperatures, and durations, while also assessing the antibacterial activity of pyocyanin against pathogens. Our aim was to understand the factors influencing pyocyanin production and its therapeutic potential For this study, we obtained 26 strains of Pseudomonas aeruginosa from various sources. Out of these, 17 strains had the ability to produce pigment. We selected the strain that demonstrated the highest production of pigment for all subsequent experiments. To investigate the influence of culture media on pyocyanin, we cultured the selected strain on various media types, including nutrient agar, Brain Heart Infusion agar, tryptic soy agar, CT agar, Muller Hinton, Nutrient broth, Peptone water, and Tryptic Soy broth. The incubation conditions were varied by testing at both 37°C and 35°C and at different time intervals (24, 48, 72, and 96 hours), as well as at different pH levels (7.5 and 7.8). Following culture growth, we extracted the pyocyanin pigment and examined its antibacterial efficacy.

@article{noauthororeditor, abstract = {We investigated the impact of culture medium on pyocyanin production by Pseudomonas aeruginosa. Our study included variations in media, temperatures, and durations, while also assessing the antibacterial activity of pyocyanin against pathogens. Our aim was to understand the factors influencing pyocyanin production and its therapeutic potential For this study, we obtained 26 strains of Pseudomonas aeruginosa from various sources. Out of these, 17 strains had the ability to produce pigment. We selected the strain that demonstrated the highest production of pigment for all subsequent experiments. To investigate the influence of culture media on pyocyanin, we cultured the selected strain on various media types, including nutrient agar, Brain Heart Infusion agar, tryptic soy agar, CT agar, Muller Hinton, Nutrient broth, Peptone water, and Tryptic Soy broth. The incubation conditions were varied by testing at both 37°C and 35°C and at different time intervals (24, 48, 72, and 96 hours), as well as at different pH levels (7.5 and 7.8). Following culture growth, we extracted the pyocyanin pigment and examined its antibacterial efficacy.}, added-at = {2024-02-16T12:35:24.000+0100}, author = {Saleh, Zainab Agab Altaee | Karama Tahreer Ahmed Altaee | Eman Naji}, biburl = {https://www.bibsonomy.org/bibtex/2e3f811017b4ce97f7016edeb826dfb67/centralasian_20}, interhash = {ce123647721069ba7de6043b711a2c2e}, intrahash = {e3f811017b4ce97f7016edeb826dfb67}, issn = {2660-4159}, journal = {CENTRAL ASIAN JOURNAL OF MEDICAL AND NATURAL SCIENCE}, keywords = {Pyocyanin antibacterial efficacy production,}, language = {english}, month = dec, number = 6, pages = {988-994}, timestamp = {2024-02-16T12:35:24.000+0100}, title = {Comparative Analysis of Pyocyanin Production and Antibacterial Efficacy on Diverse Culture}, url = {https://cajmns.centralasianstudies.org/index.php/CAJMNS/article/view/2174/2215}, volume = 4, year = 2023 }