Effects of adenosine on histamine release from human lung fragments
I. Ott, M. Lohse, K. Klotz, I. Vogt-Moykopf, and U. Schwabe. Int Arch Allergy Immunol, 98 (1):
50-6(1992)Ott, I Lohse, M J Klotz, K N Vogt-Moykopf, I Schwabe, U In Vitro
Switzerland International archives of allergy and immunology Int
Arch Allergy Immunol. 1992;98(1):50-6..
Abstract
The actions of adenosine on histamine release of human lung fragments
were investigated. Histamine release was stimulated either with the
calcium ionophore A23187 or with concanavalin A. Adenosine and its
analogue 5'-N-ethylcarboxamidoadenosine alone had no significant
effect on basal release or on the release elicited by A 23187 or
concanavalin A. However, in the presence of the adenosine receptor
antagonist 8-4-(2-aminoethyl)amino-carbonylmethyloxy-phenyl-1,
3-dipropylxanthine (XAC), which itself did not affect the release,
adenosine increased the stimulated histamine release. On the other
hand, in the presence of the nucleoside transport inhibitor S-(p-nitrobenzyl)-6-thioinosine
(NBTI), adenosine caused a reduction in stimulated histamine release.
NBTI itself caused a stimulation of release. Thus, a stimulatory
effect of adenosine was seen in the presence of XAC, whereas an inhibitory
effect was unmasked by NBTI. From these data it is concluded that
adenosine exerts two opposing effects on histamine release in the
human lung which neutralize each other: it inhibits release via a
site antagonized by XAC, which presumably represents an A2 adenosine
receptor, and it stimulates release via a mechanism that is blocked
by NBTI, suggesting that adenosine needs to reach the interior of
cells to exert this effect. The slight stimulatory effect of NBTI
alone demonstrates that trapping intracellularly formed adenosine
inside mast cells leads to sufficient concentrations of adenosine
to stimulate histamine release. These findings suggest an important
bimodal role of adenosine in regulating histamine release in the
human lung.
Ott, I Lohse, M J Klotz, K N Vogt-Moykopf, I Schwabe, U In Vitro
Switzerland International archives of allergy and immunology Int
Arch Allergy Immunol. 1992;98(1):50-6.
%0 Journal Article
%1 Ott1992
%A Ott, I.
%A Lohse, M. J.
%A Klotz, K. N.
%A Vogt-Moykopf, I.
%A Schwabe, U.
%D 1992
%J Int Arch Allergy Immunol
%K & A/administration Adenosine-5'-(N-ethylcarboxamide) Adenosine/analogs Calcimycin/pharmacology Carrier Cells/*drug Concanavalin Histamine Humans Lung/*cytology Mast Membrane Nucleoside Proteins Proteins/drug Purinergic/drug Release/*drug Thioinosine/analogs Transport derivatives/*pharmacology derivatives/pharmacology dosage effects Receptor
%N 1
%P 50-6
%T Effects of adenosine on histamine release from human lung fragments
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1378041
%V 98
%X The actions of adenosine on histamine release of human lung fragments
were investigated. Histamine release was stimulated either with the
calcium ionophore A23187 or with concanavalin A. Adenosine and its
analogue 5'-N-ethylcarboxamidoadenosine alone had no significant
effect on basal release or on the release elicited by A 23187 or
concanavalin A. However, in the presence of the adenosine receptor
antagonist 8-4-(2-aminoethyl)amino-carbonylmethyloxy-phenyl-1,
3-dipropylxanthine (XAC), which itself did not affect the release,
adenosine increased the stimulated histamine release. On the other
hand, in the presence of the nucleoside transport inhibitor S-(p-nitrobenzyl)-6-thioinosine
(NBTI), adenosine caused a reduction in stimulated histamine release.
NBTI itself caused a stimulation of release. Thus, a stimulatory
effect of adenosine was seen in the presence of XAC, whereas an inhibitory
effect was unmasked by NBTI. From these data it is concluded that
adenosine exerts two opposing effects on histamine release in the
human lung which neutralize each other: it inhibits release via a
site antagonized by XAC, which presumably represents an A2 adenosine
receptor, and it stimulates release via a mechanism that is blocked
by NBTI, suggesting that adenosine needs to reach the interior of
cells to exert this effect. The slight stimulatory effect of NBTI
alone demonstrates that trapping intracellularly formed adenosine
inside mast cells leads to sufficient concentrations of adenosine
to stimulate histamine release. These findings suggest an important
bimodal role of adenosine in regulating histamine release in the
human lung.
@article{Ott1992,
abstract = {The actions of adenosine on histamine release of human lung fragments
were investigated. Histamine release was stimulated either with the
calcium ionophore A23187 or with concanavalin A. Adenosine and its
analogue 5'-N-ethylcarboxamidoadenosine alone had no significant
effect on basal release or on the release elicited by A 23187 or
concanavalin A. However, in the presence of the adenosine receptor
antagonist 8-[4-[[[[(2-aminoethyl)amino]-carbonyl]methyloxy]-phenyl]-1,
3-dipropylxanthine (XAC), which itself did not affect the release,
adenosine increased the stimulated histamine release. On the other
hand, in the presence of the nucleoside transport inhibitor S-(p-nitrobenzyl)-6-thioinosine
(NBTI), adenosine caused a reduction in stimulated histamine release.
NBTI itself caused a stimulation of release. Thus, a stimulatory
effect of adenosine was seen in the presence of XAC, whereas an inhibitory
effect was unmasked by NBTI. From these data it is concluded that
adenosine exerts two opposing effects on histamine release in the
human lung which neutralize each other: it inhibits release via a
site antagonized by XAC, which presumably represents an A2 adenosine
receptor, and it stimulates release via a mechanism that is blocked
by NBTI, suggesting that adenosine needs to reach the interior of
cells to exert this effect. The slight stimulatory effect of NBTI
alone demonstrates that trapping intracellularly formed adenosine
inside mast cells leads to sufficient concentrations of adenosine
to stimulate histamine release. These findings suggest an important
bimodal role of adenosine in regulating histamine release in the
human lung.},
added-at = {2010-12-14T18:12:02.000+0100},
author = {Ott, I. and Lohse, M. J. and Klotz, K. N. and Vogt-Moykopf, I. and Schwabe, U.},
biburl = {https://www.bibsonomy.org/bibtex/2f8470208d34d706ce728ebd8d731d0c2/pharmawuerz},
endnotereftype = {Journal Article},
interhash = {59a8896ef05ed3e9116f3ca8989460b4},
intrahash = {f8470208d34d706ce728ebd8d731d0c2},
issn = {1018-2438 (Print) 1018-2438 (Linking)},
journal = {Int Arch Allergy Immunol},
keywords = {& A/administration Adenosine-5'-(N-ethylcarboxamide) Adenosine/analogs Calcimycin/pharmacology Carrier Cells/*drug Concanavalin Histamine Humans Lung/*cytology Mast Membrane Nucleoside Proteins Proteins/drug Purinergic/drug Release/*drug Thioinosine/analogs Transport derivatives/*pharmacology derivatives/pharmacology dosage effects Receptor},
note = {Ott, I Lohse, M J Klotz, K N Vogt-Moykopf, I Schwabe, U In Vitro
Switzerland International archives of allergy and immunology Int
Arch Allergy Immunol. 1992;98(1):50-6.},
number = 1,
pages = {50-6},
shorttitle = {Effects of adenosine on histamine release from human lung fragments},
timestamp = {2010-12-14T18:20:07.000+0100},
title = {Effects of adenosine on histamine release from human lung fragments},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1378041},
volume = 98,
year = 1992
}