Cnidoscolus chayamansa is a plant widely used as food with potential medicinal applications. Plant tissue culture enables the production of secondary metabolites from plants in a sustainable manner. The aim of this work was to establish a callus culture of C. chayamansa using leaf explants, which, in the future, can be used to obtain whole extracts or specific compounds of interest. Different concentrations and combinations of 6-Benzylaminopurine (BAP) and 2,4-Dichlorophenoxyacetic Acid (2,4-D) in Murashige and Skoog (MS) culture medium were evaluated for their ability to support callus induction from immature leaf explants. Highest callus yield (100%) was achieved when leaf explants were incubated in MS medium supplemented with BAP (5.0 mg L-1) and 2,4-D (2.5 or 5.0 mg mL-1). A reduced rate of callus induction (75%) was observed when leaf explants were exposed to lower concentrations (0.5 mg L-1) of BAP or 2,4-D. The calluses obtained in this work can be used as a biotechnological alternative for in vitro propagation of C. chayamansa and for obtaining extracts or compounds with a possible pharmacological application.
%0 Journal Article
%1 noauthororeditor2018establishing
%A Pérez-González, Mariana Zuleima
%A Nieto-Trujillo, Aurelio
%A García-Martínez, Ignacio
%A Estrada-Zúñiga, María Elena
%A Bernabé-Antonio, Antonio
%A Jimenez-Arellanes, María Adelina
%A Cruz-Sosa, Francisco
%D 2018
%J Advances in Biochemistry and Biotechnology
%K Chaya In Medicinal Plant culture growth plants regulators vitro
%N 02
%P 1-5
%T "Establishing a Callus Culture of Cnidoscolus chayamansa McVaugh: A Species with Ethnopharmacological Value"
%U https://gavinpublishers.com/journals/artical_in_press/advances-in-biochemistry-and-biotechnology-ISSN-2574-7258
%V 2018
%X Cnidoscolus chayamansa is a plant widely used as food with potential medicinal applications. Plant tissue culture enables the production of secondary metabolites from plants in a sustainable manner. The aim of this work was to establish a callus culture of C. chayamansa using leaf explants, which, in the future, can be used to obtain whole extracts or specific compounds of interest. Different concentrations and combinations of 6-Benzylaminopurine (BAP) and 2,4-Dichlorophenoxyacetic Acid (2,4-D) in Murashige and Skoog (MS) culture medium were evaluated for their ability to support callus induction from immature leaf explants. Highest callus yield (100%) was achieved when leaf explants were incubated in MS medium supplemented with BAP (5.0 mg L-1) and 2,4-D (2.5 or 5.0 mg mL-1). A reduced rate of callus induction (75%) was observed when leaf explants were exposed to lower concentrations (0.5 mg L-1) of BAP or 2,4-D. The calluses obtained in this work can be used as a biotechnological alternative for in vitro propagation of C. chayamansa and for obtaining extracts or compounds with a possible pharmacological application.
@article{noauthororeditor2018establishing,
abstract = {Cnidoscolus chayamansa is a plant widely used as food with potential medicinal applications. Plant tissue culture enables the production of secondary metabolites from plants in a sustainable manner. The aim of this work was to establish a callus culture of C. chayamansa using leaf explants, which, in the future, can be used to obtain whole extracts or specific compounds of interest. Different concentrations and combinations of 6-Benzylaminopurine (BAP) and 2,4-Dichlorophenoxyacetic Acid (2,4-D) in Murashige and Skoog (MS) culture medium were evaluated for their ability to support callus induction from immature leaf explants. Highest callus yield (100%) was achieved when leaf explants were incubated in MS medium supplemented with BAP (5.0 mg L-1) and 2,4-D (2.5 or 5.0 mg mL-1). A reduced rate of callus induction (75%) was observed when leaf explants were exposed to lower concentrations (0.5 mg L-1) of BAP or 2,4-D. The calluses obtained in this work can be used as a biotechnological alternative for in vitro propagation of C. chayamansa and for obtaining extracts or compounds with a possible pharmacological application. },
added-at = {2019-05-02T09:21:04.000+0200},
author = {Pérez-González, Mariana Zuleima and Nieto-Trujillo, Aurelio and García-Martínez, Ignacio and Estrada-Zúñiga, María Elena and Bernabé-Antonio, Antonio and Jimenez-Arellanes, María Adelina and Cruz-Sosa, Francisco},
biburl = {https://www.bibsonomy.org/bibtex/2908b4447cc92394cd7432afb6438f280/alyssacarter},
interhash = {7beb45640a48e0f02f6a8c3ed8ebae7c},
intrahash = {908b4447cc92394cd7432afb6438f280},
issn = {ISSN: 2574-7258},
journal = {Advances in Biochemistry and Biotechnology },
keywords = {Chaya In Medicinal Plant culture growth plants regulators vitro},
language = {English},
month = {April },
number = 02,
pages = {1-5},
timestamp = {2019-05-02T09:21:04.000+0200},
title = {"Establishing a Callus Culture of Cnidoscolus chayamansa McVaugh: A Species with Ethnopharmacological Value"},
url = {https://gavinpublishers.com/journals/artical_in_press/advances-in-biochemistry-and-biotechnology-ISSN-2574-7258},
volume = 2018,
year = 2018
}