Purification and functional characterization of beta-adrenergic receptor
kinase expressed in insect cells
P. Sohlemann, M. Hekman, C. Buchen, J. Elce, and M. Lohse. FEBS Lett, 324 (1):
59-62(June 1993)Sohlemann, P Hekman, M Buchen, C Elce, J S Lohse, M J Comparative
Study Research Support, Non-U.S. Gov't Netherlands FEBS letters FEBS
Lett. 1993 Jun 7;324(1):59-62..
Abstract
The beta-adrenergic receptor kinase mediates agonist-dependent phosphorylation
of beta-adrenergic receptors, which is thought to represent the first
step of homologous desensitization. We have expressed bovine and
human beta ARK1 in Sf9 cells and purified them to apparent homogeneity
in milligram quantities. The Km-values of the enzyme were 3.8 microM
for rhodopsin and 22 microM for ATP; the Vmax-value was 9.9 mol phosphate/mol
beta ARK/min. These data indicate that the two recombinant kinases
were at least as active as preparations previously obtained from
bovine brain. There were no differences in the functional activity
of human and bovine beta ARK.
Sohlemann, P Hekman, M Buchen, C Elce, J S Lohse, M J Comparative
Study Research Support, Non-U.S. Gov't Netherlands FEBS letters FEBS
Lett. 1993 Jun 7;324(1):59-62.
%0 Journal Article
%1 Sohlemann1993
%A Sohlemann, P.
%A Hekman, M.
%A Buchen, C.
%A Elce, J. S.
%A Lohse, M. J.
%D 1993
%J FEBS Lett
%K & *Cyclic AMP-Dependent Adenosine Animals Cell Chromatography, Cloning, Exchange Gel Humans Ion Kinases Kinases/genetics/*isolation Kinetics Line Molecular Moths Outer Phosphorylation Protein Proteins/isolation Receptor Recombinant Rhodopsin/metabolism Rod Segment/metabolism Transfection Triphosphate/metabolism beta-Adrenergic purification/*metabolism purification/metabolism
%N 1
%P 59-62
%T Purification and functional characterization of beta-adrenergic receptor
kinase expressed in insect cells
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8504860
%V 324
%X The beta-adrenergic receptor kinase mediates agonist-dependent phosphorylation
of beta-adrenergic receptors, which is thought to represent the first
step of homologous desensitization. We have expressed bovine and
human beta ARK1 in Sf9 cells and purified them to apparent homogeneity
in milligram quantities. The Km-values of the enzyme were 3.8 microM
for rhodopsin and 22 microM for ATP; the Vmax-value was 9.9 mol phosphate/mol
beta ARK/min. These data indicate that the two recombinant kinases
were at least as active as preparations previously obtained from
bovine brain. There were no differences in the functional activity
of human and bovine beta ARK.
@article{Sohlemann1993,
abstract = {The beta-adrenergic receptor kinase mediates agonist-dependent phosphorylation
of beta-adrenergic receptors, which is thought to represent the first
step of homologous desensitization. We have expressed bovine and
human beta ARK1 in Sf9 cells and purified them to apparent homogeneity
in milligram quantities. The Km-values of the enzyme were 3.8 microM
for rhodopsin and 22 microM for ATP; the Vmax-value was 9.9 mol phosphate/mol
beta ARK/min. These data indicate that the two recombinant kinases
were at least as active as preparations previously obtained from
bovine brain. There were no differences in the functional activity
of human and bovine beta ARK.},
added-at = {2010-12-14T18:12:02.000+0100},
author = {Sohlemann, P. and Hekman, M. and Buchen, C. and Elce, J. S. and Lohse, M. J.},
biburl = {https://www.bibsonomy.org/bibtex/2054134af7cf23973654809c6a198e3d1/pharmawuerz},
endnotereftype = {Journal Article},
interhash = {100efefa822dcabef5a65d3f19b2dd43},
intrahash = {054134af7cf23973654809c6a198e3d1},
issn = {0014-5793 (Print) 0014-5793 (Linking)},
journal = {FEBS Lett},
keywords = {& *Cyclic AMP-Dependent Adenosine Animals Cell Chromatography, Cloning, Exchange Gel Humans Ion Kinases Kinases/genetics/*isolation Kinetics Line Molecular Moths Outer Phosphorylation Protein Proteins/isolation Receptor Recombinant Rhodopsin/metabolism Rod Segment/metabolism Transfection Triphosphate/metabolism beta-Adrenergic purification/*metabolism purification/metabolism},
month = {Jun 7},
note = {Sohlemann, P Hekman, M Buchen, C Elce, J S Lohse, M J Comparative
Study Research Support, Non-U.S. Gov't Netherlands FEBS letters FEBS
Lett. 1993 Jun 7;324(1):59-62.},
number = 1,
pages = {59-62},
shorttitle = {Purification and functional characterization of beta-adrenergic receptor
kinase expressed in insect cells},
timestamp = {2010-12-14T18:22:03.000+0100},
title = {Purification and functional characterization of beta-adrenergic receptor
kinase expressed in insect cells},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8504860},
volume = 324,
year = 1993
}