Mycorrhizal lipochitinoligosaccharides (LCOs) depolarize root hairs of Medicago truncatula
A. Hurter, S. Fort, S. Cottaz, R. Hedrich, D. Geiger, and M. Roelfsema. PLoS One, 13 (5):
e0198126(2018)Hurter, Anna-Lena
Fort, Sebastien
Cottaz, Sylvain
Hedrich, Rainer
Geiger, Dietmar
Roelfsema, M Rob G
eng
Research Support, Non-U.S. Gov't
2018/06/01
PLoS One. 2018 May 31;13(5):e0198126. doi: 10.1371/journal.pone.0198126. eCollection 2018..
DOI: 10.1371/journal.pone.0198126
Abstract
Arbuscular Mycorrhiza and Root Nodule Symbiosis are symbiotic interactions with a high benefit for plant growth and crop production. Thus, it is of great interest to understand the developmental process of these symbioses in detail. We analysed very early symbiotic responses of Medicago truncatula root hair cells, by stimulation with lipochitinoligosaccharides specific for the induction of nodules (Nod-LCOs), or the interaction with mycorrhiza (Myc-LCOs). Intracellular micro electrodes were used, in combination with Ca2+ sensitive reporter dyes, to study the relations between cytosolic Ca2+ signals and membrane potential changes. We found that sulfated Myc- as well as Nod-LCOs initiate a membrane depolarization, which depends on the chemical composition of these signaling molecules, as well as the genotype of the plants that were studied. A successive application of sulfated Myc-LCOs and Nod-LCOs resulted only in a single transient depolarization, indicating that Myc-LCOs can repress plasma membrane responses to Nod-LCOs. In contrast to current models, the Nod-LCO-induced depolarization precedes changes in the cytosolic Ca2+ level of root hair cells. The Nod-LCO induced membrane depolarization thus is most likely independent of cytosolic Ca2+ signals and nuclear Ca2+ spiking.
Hurter, Anna-Lena
Fort, Sebastien
Cottaz, Sylvain
Hedrich, Rainer
Geiger, Dietmar
Roelfsema, M Rob G
eng
Research Support, Non-U.S. Gov't
2018/06/01
PLoS One. 2018 May 31;13(5):e0198126. doi: 10.1371/journal.pone.0198126. eCollection 2018.
%0 Journal Article
%1 hurter2018mycorrhizal
%A Hurter, A. L.
%A Fort, S.
%A Cottaz, S.
%A Hedrich, R.
%A Geiger, D.
%A Roelfsema, M. R. G.
%D 2018
%J PLoS One
%K Cell Membrane/drug effects/metabolism myOwn
%N 5
%P e0198126
%R 10.1371/journal.pone.0198126
%T Mycorrhizal lipochitinoligosaccharides (LCOs) depolarize root hairs of Medicago truncatula
%U https://www.ncbi.nlm.nih.gov/pubmed/29851976
%V 13
%X Arbuscular Mycorrhiza and Root Nodule Symbiosis are symbiotic interactions with a high benefit for plant growth and crop production. Thus, it is of great interest to understand the developmental process of these symbioses in detail. We analysed very early symbiotic responses of Medicago truncatula root hair cells, by stimulation with lipochitinoligosaccharides specific for the induction of nodules (Nod-LCOs), or the interaction with mycorrhiza (Myc-LCOs). Intracellular micro electrodes were used, in combination with Ca2+ sensitive reporter dyes, to study the relations between cytosolic Ca2+ signals and membrane potential changes. We found that sulfated Myc- as well as Nod-LCOs initiate a membrane depolarization, which depends on the chemical composition of these signaling molecules, as well as the genotype of the plants that were studied. A successive application of sulfated Myc-LCOs and Nod-LCOs resulted only in a single transient depolarization, indicating that Myc-LCOs can repress plasma membrane responses to Nod-LCOs. In contrast to current models, the Nod-LCO-induced depolarization precedes changes in the cytosolic Ca2+ level of root hair cells. The Nod-LCO induced membrane depolarization thus is most likely independent of cytosolic Ca2+ signals and nuclear Ca2+ spiking.
@article{hurter2018mycorrhizal,
abstract = {Arbuscular Mycorrhiza and Root Nodule Symbiosis are symbiotic interactions with a high benefit for plant growth and crop production. Thus, it is of great interest to understand the developmental process of these symbioses in detail. We analysed very early symbiotic responses of Medicago truncatula root hair cells, by stimulation with lipochitinoligosaccharides specific for the induction of nodules (Nod-LCOs), or the interaction with mycorrhiza (Myc-LCOs). Intracellular micro electrodes were used, in combination with Ca2+ sensitive reporter dyes, to study the relations between cytosolic Ca2+ signals and membrane potential changes. We found that sulfated Myc- as well as Nod-LCOs initiate a membrane depolarization, which depends on the chemical composition of these signaling molecules, as well as the genotype of the plants that were studied. A successive application of sulfated Myc-LCOs and Nod-LCOs resulted only in a single transient depolarization, indicating that Myc-LCOs can repress plasma membrane responses to Nod-LCOs. In contrast to current models, the Nod-LCO-induced depolarization precedes changes in the cytosolic Ca2+ level of root hair cells. The Nod-LCO induced membrane depolarization thus is most likely independent of cytosolic Ca2+ signals and nuclear Ca2+ spiking.},
added-at = {2024-02-15T15:08:22.000+0100},
author = {Hurter, A. L. and Fort, S. and Cottaz, S. and Hedrich, R. and Geiger, D. and Roelfsema, M. R. G.},
biburl = {https://www.bibsonomy.org/bibtex/202fbaa5cdedfb46bda13b414605580ba/jvsi_all},
doi = {10.1371/journal.pone.0198126},
interhash = {f318d90bd332945c19ecf9599840c77c},
intrahash = {02fbaa5cdedfb46bda13b414605580ba},
issn = {1932-6203 (Electronic)
1932-6203 (Linking)},
journal = {PLoS One},
keywords = {Cell Membrane/drug effects/metabolism myOwn},
note = {Hurter, Anna-Lena
Fort, Sebastien
Cottaz, Sylvain
Hedrich, Rainer
Geiger, Dietmar
Roelfsema, M Rob G
eng
Research Support, Non-U.S. Gov't
2018/06/01
PLoS One. 2018 May 31;13(5):e0198126. doi: 10.1371/journal.pone.0198126. eCollection 2018.},
number = 5,
pages = {e0198126},
timestamp = {2024-02-15T15:08:22.000+0100},
title = {Mycorrhizal lipochitinoligosaccharides (LCOs) depolarize root hairs of Medicago truncatula},
type = {Journal Article},
url = {https://www.ncbi.nlm.nih.gov/pubmed/29851976},
volume = 13,
year = 2018
}