%0 Generic %1 nakasono2013intermediate %A NAKASONO, S %A TAKAHASHI, M %A NEGISHI, T %A IKEHATA, M %A YOSHIE, S %D 2013 %K Carcinogenicity Field Frequency Genotoxicity Intermediate Magnetic Promotion Test in vitro %T Intermediate Frequency Magnetic Fields Did Not Have Genotoxicor Promotion Potentials In in vitroAssay %X In contrast to extremely low frequency and radio frequency electromagnetic fields (EMF), the biological effects ofintermediate frequency (IF; 300Hz to 1 OMHz) EMF have not been studied very well. In this study, we have investigated the effect ofIF magnetic fields (MFs)on genotoxicity and promotion potentialsin animal or human cells, by using in vitro micronucleus formation test (MN), mouse lymphoma assay (MLA), chromosomal aberration test (CA) and transformation assay. For the in vitro research, we used a Helmholtz type exposure system which can generate vertical and sinusoidal MFs, such as 0.9lmT at 2 kHz, l.lmT at 20 kHz and 0.1 lmT at 60 kHz.For the MN, we used the Chinese hamster derived cell line, V79. The effects on the repair system of DNA damage caused by mitomycin C (MMC) were also tested. Rates of micronucleus formation were determined as the proportion of binucleus cells with micronucleus to the total number of binucleus cells. For the MLA, we used a mouse lymphoma cell line, L5178Y tk+/- 3.7.2c. The effects on the repair system of DNA damage caused by methylmethanesulfonate (MMS) were also tested by the MLA. For the CA, we used a human lymphoblastoid cell line, TK6, which has normal p53 DNA repair system. The effects on the human normal DNA repair system by using MMC were also tested. For transformation assay, we used a Bhas 42 cell, which was established from Balb/c 3T3 cells transfected with v-Ha-ras oncogene. The co-promotion effect was also evaluated by using TP A, as a known tumor promoter. In statistical analysis for all above tests, neither significant nor reproducible difference was found between exposed and control groups. These results indicated that the strong IF MFs used in this study did not induce micronucleus formation, point mutation, chromosomal aberration or transformation, and did not affect DNA damage by MMC or MMS, or DNA damage repair system, or co-promotion activity to TPA, in animal or human cells.

@conference{nakasono2013intermediate, abstract = {In contrast to extremely low frequency and radio frequency electromagnetic fields (EMF), the biological effects ofintermediate frequency (IF; 300Hz to 1 OMHz) EMF have not been studied very well. In this study, we have investigated the effect ofIF magnetic fields (MFs)on genotoxicity and promotion potentialsin animal or human cells, by using in vitro micronucleus formation test (MN), mouse lymphoma assay (MLA), chromosomal aberration test (CA) and transformation assay. For the in vitro research, we used a Helmholtz type exposure system which can generate vertical and sinusoidal MFs, such as 0.9lmT at 2 kHz, l.lmT at 20 kHz and 0.1 lmT at 60 kHz.For the MN, we used the Chinese hamster derived cell line, V79. The effects on the repair system of DNA damage caused by mitomycin C (MMC) were also tested. Rates of micronucleus formation were determined as the proportion of binucleus cells with micronucleus to the total number of binucleus cells. For the MLA, we used a mouse lymphoma cell line, L5178Y tk+/- 3.7.2c. The effects on the repair system of DNA damage caused by methylmethanesulfonate (MMS) were also tested by the MLA. For the CA, we used a human lymphoblastoid cell line, TK6, which has normal p53 DNA repair system. The effects on the human normal DNA repair system by using MMC were also tested. For transformation assay, we used a Bhas 42 cell, which was established from Balb/c 3T3 cells transfected with v-Ha-ras oncogene. The co-promotion effect was also evaluated by using TP A, as a known tumor promoter. In statistical analysis for all above tests, neither significant nor reproducible difference was found between exposed and control groups. These results indicated that the strong IF MFs used in this study did not induce micronucleus formation, point mutation, chromosomal aberration or transformation, and did not affect DNA damage by MMC or MMS, or DNA damage repair system, or co-promotion activity to TPA, in animal or human cells.}, added-at = {2021-02-12T13:05:42.000+0100}, author = {NAKASONO, S and TAKAHASHI, M and NEGISHI, T and IKEHATA, M and YOSHIE, S}, biburl = {https://www.bibsonomy.org/bibtex/2ea702dc1e3370ee9e001ff6c0f34eeed/ceps}, interhash = {29cda23dc5342797fef71ccc1c6eb4e4}, intrahash = {ea702dc1e3370ee9e001ff6c0f34eeed}, keywords = {Carcinogenicity Field Frequency Genotoxicity Intermediate Magnetic Promotion Test in vitro}, timestamp = {2023-12-21T15:03:17.000+0100}, title = {Intermediate Frequency Magnetic Fields Did Not Have Genotoxicor Promotion Potentials In in vitroAssay}, year = 2013 }